uid=AND,o=lter,dc=ecoinformatics,dc=org all public read HS006 Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Principal Investigator Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Creator 2011-09-14 The biochemical characteristics of fine benthic organic matter (FBOM) were studied in 14 stream reaches. Half of these low-order mountain stream reaches had experienced significant debris flow events during server winter storms and half did not. stream ecology debris flows organic matter sediments forest ecosystems aquatic ecosystems streams LTER controlled vocabulary organic matter LTER core research areas Bonin, L. H., R. P. Griffiths, and B. A. Caldwell. 1999. Effects of storage on measurements of potential microbial activities in stream fine benthic organic matter. J. Microb. Methods 38:91-99. Bonin, L. H., R. P. Griffiths, and B. A. Caldwell. 2000. Nutrient and microbial characteristics of fine benthic organic matter in mountain streams. J. North Amer. Benth. Soc. 19:235-249. Bonin, L. H., R. P. Griffiths, and B. A. Caldwell. Spatiotemporal nutrient distribution of fine particulate organic matter in low-order Oregon Cascade streams. Soil Biol. Biochem. (in review 5/01). Students who conducted measurements at the H.J. Andrews during the summer of 1996: Alan Swanson, Paul Graphton, Amy Rousseau, Jodi Garton, and Thomas Barnes. Alan Swanson was the field research team supervisor. Lousia Hooven conducted some of the laboratory analyses. The National Science Foundation provided financial support from grants BSR-9011663, BIO-9200809, and DEB-9318502 from the Long-Term Ecological Research program.Related FilesTitle: Site DescriptionsDescription: Physical characteristics of sitesURL: https://andrewsforest.oregonstate.edu/data/studies/hs006/hs006_sitedescription.pdf Data Use Agreement: The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. This Data Set is released under the Creative Commons license CC BY "Attribution" (see: https://creativecommons.org/licenses/by/4.0/). Creative Commons license CC BY - Attribution is a license that allows others to distribute, remix, tweak, and build upon your work (even commercially), as long as you are credited for the original creation. This license accommodates maximum dissemination and use of licensed materials. It is considered professional conduct and an ethical obligation to acknowledge the work of other scientists. The Data User is asked to provide attribution of the original work if this data package is shared in whole or by individual parts or used in the derivation of other products. A recommended citation is provided for each Data Set in the Andrews LTER data catalog (see: http://andlter.forestry.oregonstate.edu/data/catalog/datacatalog.aspx). A generic citation is also provided for this Data Set on the website https://portal.edirepository.org in the summary metadata page. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed and all data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. General acknowledgement: Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. If data used in publication, the PI will be listed as a coauthor. Whenever these data are presented in whatever form, the PI will be acknowledged. http://andlter.forestry.oregonstate.edu/data/abstract.aspx?dbcode=HS006 1996-06-26 1996-09-06 The biochemical characteristics of fine benthic organic matter (FBOM) were studied in 14 stream reaches. Half of these low-order mountain stream reaches had experienced significant debris flow events during server winter storms and half did not.

An update history is logged and maintained with each new version of every dataset.

notPlanned Original metadata creation Version1 2001-04-23 Moved database to SQL Server Version2 2005-06-27 Information Manager

Andrews Forest LTER Program US Forest Service Pacific Northwest Research Station 3200 SW Jefferson Way Corvallis OR 97331

hjaweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Andrews Forest LTER Site

Forest Ecosystems and Society Department in Forestry Oregon State University 201K Richardson Hall Corvallis OR 97331-5752

(541) 737-8480 lterweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Field Methods - HS006 Sediments were sampled on 4 occasions: 6/26, 7/1, 7/30, 9/6 1996. FBOM was collected from stream beds with a hand vacuum pump into a 2 L collecting jar. The intake line was fitted with a 1 mm stainless steel screen, allowing benthic material to be wet-sieved during sampling. Samples were transferred to polystyrene jars (500 mL) and stored in an insulated chest with stream water and ice. In the laboratory, a slurry was prepared by decanting excess stream water from the jars and mixing, keeping FBOM suspended while subsampling. Subsamples were dispensed using 1, 3, or 5 mL plastic syringes with enlarged openings. All laboratory analyses of slurries began immediately upon return from FBOM collection (Bonin et al., 2000) conform with known time constraints on sampling and sample processing (Bonin et al., 1999). Laboratory Methods - HS006 Denitrification potential was measured as N2O production in FBOM slurries incubated in an AR atmosphere and amended with glucose and NaNO3 (Martin et al. 1988). Duplicate 5 mL FBOM slurry samples in 25 mL Erlenmeyer flasks were capped with rubber stoppers and purged for 3 minutes with argon at 120 cc/min. The flasks were gently shaken to remove air bubble and incubated at 24°C for 1h. After this initial incubation, 2 mL of a sterile 1 mM glucose and 1 mM NaNO3 solution was injected through the stopper and 2 mL of headspace removed. Incubation was continued at 24°C for an additional 3 h. After 1 and 3 hours, a gas chromatograph (GC) equipped with an electron capture detector was used to measure N2O concentrations. Putative nitrogen fixation rates were measured by acetylene reduction (Weaver and Danso 1994). Samples were prepared as for denitrification except the headspace was replaced with 1.5% O2, 12.5% acetylene and 86% argon. After the samples had incubated for 24 h, ethylene concentrations were measured on a GC equipped with a flame ionization detector. A control without acetylene was analyzed for endogenous ethylene production. Respiration was measured on duplicate 5 mL FBOM slurry samples in 25 mL Erlenmeyer flasks capped with rubber stoppers. Slurries were incubated at 24°C for 3 h. At 1 and 3 h., the headspace was analyzed for CO2 on a GC fitted with a thermal conductivity conductor. s-glucosidase activities were measured using the spectrophotometric assay of Tabatabai and Bremner (1969), as modified by Zou et al. (1992). One mL of 10 mM p-nitrophenyl s-D glucopyranoside substrate was added to duplicate 1 mL subsamples containing suspended FBOM. The tubes were shaken and then placed in a 30°C water bath for 2 hours, along with duplicate controls with no s-glucosidase substrate addition. After incubating, 1 mL of 10 mM p-nitrophenyl s-D glucopyranoside was added to the controls and all reactions were immediately stopped with the addition of 0.5 mL of 0.5 M CaCl2 and 2 mL of 0.1 M tris[hydroxymethyl]aminomethane at pH 12.0. The mixtures were centrifuged for 5 minutes at 500 x g. From the supernatant, 0.2 mL of solution was diluted with 2.0 mL deionized water and the optical density measured at 410 nm. A standard curve was prepared from 0.02-1.0 Fmol mL-1 p-nitrophenol. Phosphatase followed the same general procedure as for s-glucosidase, except the substrate used was 1 mL of 50 mM p-nitrophenyl phosphate, incubation period was 1 hour, and 2 mL of 0.5 M NaOH instead of 0.1 M tris[hydroxymethyl]aminomethane, were added to terminate the reaction. Mineralizable nitrogen measurements were determined using the 7 d anaerobic incubation method (Keeney 1982). Duplicate 10 mL FBOM samples in 50 mL screw-topped test tubes were filled to the top edge with deionized water, capped and incubated at 40°C for 7 d. After incubation an equal amount of 4 M KCl was added, shaken for 1 hour in the presence of 0.4 mL 10M NaOH and analyzed for NH4-N using a selective ion electrode (Corning ammonium electrode, Medford, MA). The value for extractable ammonium was subtracted from the total to account for background levels of ammonium prior to incubation. Extractable ammonium was extracted by adding 50 mL of 2 M KCl to duplicate 10 mL samples in 250 mL Erlenmeyer flasks. Flasks were capped, shaken while incubating for 1 hour in the presence of 0.4 mL 10 M NaOH and analyzed using a selective ion electrode to determine KCl-extractable ammonium concentration. Sampling frequency: Four monthly samples at each site. We wanted to determine the effects of recent debris flows on the biochemical characteristics of small stream fine benthic organic matter (FBOM). Seven pairs of streams were used; half had experienced debris flows during the late winter storms and half did not. Andrews Experimental Forest (HJA) -122.26172200 -122.10084700 44.28196400 44.19770400 1631 1631 meter Sherri L. Johnson

US Forest Service ;Pacific NW Research Station ;3200 SW Jefferson Way Corvallis OR 97331 USA

541-758-7771 sherri.johnson2@usda.gov sherri.johnson@oregonstate.edu https://www.fs.fed.us/research/people/profile.php?alias=sherrijohnson Principal Investigator Julia A. Jones

Oregon State University;Department of Geosciences; Wilkinson Hall 104 Corvallis OR 97331-5506 USA

(541) 737-1224 Julia.Jones@oregonstate.edu geojulia@comcast.net http://ceoas.oregonstate.edu/profile/jones/ http://orcid.org/0000-0001-9429-8925 Principal Investigator Matthew G Betts

Department of Forest Ecosystems and Society; 201E Richardson Hall; College of Forestry; Oregon State University Corvallis OR 97331

(541) 737-3841 matt.betts@oregonstate.edu http://www.fsl.orst.edu/flel/index.htm Principal Investigator Michael P. Nelson

Department of Forest Ecosystems and Society; 201K Richarson Hall; College of Forestry; Oregon State University Corvallis OR 97331

541-737-9221 mpnelson@oregonstate.edu http://www.michaelpnelson.com http://orcid.org/0000-0001-6917-4752 Principal Investigator David Bell david.bell@usda.gov david.bell@oregonstate.edu https://lemma.forestry.oregonstate.edu/about/david-bell Principal Investigator The H.J. Andrews Experimental Forest is a living laboratory that provides unparalleled opportunities for the study of forest and stream ecosystems in the central Cascade Range of Oregon. Since 1980, as a part of the National Science Foundation Long Term Ecological Research (NSF-LTER) program, the Andrews Experimental Forest has become a leader in the analysis of forest and stream ecosystem dynamics. Long-term field experiments and measurement programs have focused on climate dynamics, streamflow, water quality, and vegetation succession. Currently researchers are working to develop concepts and tools needed to predict effects of natural disturbance, land use, and climate change on ecosystem structure, function, and species composition. The Andrews Experimental Forest is administered cooperatively by the USDA Forest Service Pacific Northwest Research Station, Oregon State University and the Willamette National Forest. Funding for the research program comes from the National Science Foundation (NSF), US Forest Service Pacific Northwest Research Station, Oregon State University, and other sources. Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. National Science Foundation: DEB1440409 The Andrews Forest is situated in the western Cascade Range of Oregon, and covers the entire 15,800-acre (6400-ha) drainage basin of Lookout Creek. Elevation ranges from 1350 to 5340 feet (410 to 1630 m). Broadly representative of the rugged mountainous landscape of the Pacific Northwest, the Andrews Forest contains excellent examples of the region's conifer forests and associated wildlife and stream ecosystems. These forests are among the tallest and most productive in the world, with tree heights of often greater than 250 ft (75 m). Streams are steep, cold and clean, providing habitat for numerous aquatic organisms. 1 HS00601 1996 Stream pulse data: Biochemical analysis of fine benthic organic matter in streams experiencing debris flows compared with those with no flows. HS00601.csv 5707 30a0893a4b3cc5237bfc38b4b8ecce7d 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=HS00601_v1.csv 1996-06-26 1996-09-06 DATACODE Database code char(5) Database code FORMAT Entity number numeric(1,0) number 1 natural 1.0000 1.0000 SITE Site designator char(3) Site designator CONDITN Condition of stream; was there a debris flow? (Yes, No) char(3) yes there was a debris flow no there was no debris flow PAIR Designators for paired streams; there were seven pairs with and without debris flow numeric(1,0) number 1 natural 1.0000 7.0000 DATE Sample date; 1=6/26/96; 2=7/1/96; 3=7/30/96; 4=9/6/96 char(1) 1 06/26/1996 sample 2 07/01/1996 sample 3 07/30/1996 sample 4 09/06/1996 sample TEMP Stream temperature numeric(4,1) degrees Celsius 1 real 7.0000 21.0000 RESP_OM Slurry respiration rates normalized to g organic matter numeric(6,2) micrograms per gram per hour 1 real 15.7500 165.1700 RESP Slurry respiration rates normalized to g dry mass numeric(6,2) micrograms per gram per hour 1 real 2.8600 43.9600 DENIT_OM Slurry denitrification potential normalized to g organic numeric(6,2) micrograms per gram per hour 1 real 1.4400 20.9700 DENIT Slurry denitrification potential normalized to g dry mass numeric(6,2) micrograms per gram per hour 1 real 0.2500 6.7700 N2FIX_OM Slurry nitrogen fixation normalized to g organic matter numeric(6,2) micromoles per gram per hour 1 real 0.0100 71.0600 N2FIX Slurry nitrogen fixation normalized to g dry mass numeric(6,2) micromoles per gram per hour 1 real 0.0200 76.4600 PHOSPH_OM Phosphatase activity normalized to g OM numeric(6,2) micromoles per gram per hour 1 real 10.6900 121.9100 PHOSPH Phosphatase activity normalized to g dry mass numeric(6,2) micromoles per gram per hour 1 real 1.3900 31.2700 B_GLUC_OM Beta-glucosidase activity normalized to g OM numeric(6,2) micromoles per gram per hour 1 real 2.6700 61.6400 B_GLUC Beta-glucosidase activity normalized to g dry mass numeric(6,2) micromoles per gram per hour 1 real 0.3500 17.0300 EXAMM_OM Extractable ammonium normalized to g OM numeric(5,2) micromoles per gram 1 real 1.0400 23.8300 EXAMM Extractable ammonium normalized to g dry mass numeric(5,2) micromoles per gram 1 real 0.1500 6.3400 MINN_OM Mineralizable nitrogen normalized to g OM numeric(6,2) micromoles per gram 1 real 22.7200 260.4200 MINN Mineralizable nitrogen normalized to g dry mass numeric(5,2) micromoles per gram 1 real 3.2200 69.3100 DRYWT_OM Dry weight divided by OM numeric(4,2) number 1 real 0.5600 3.9600 PRIMARY HS00601.SITE HS00601.CONDITN HS00601.PAIR HS00601.DATE NOTNULL HS00601.DATACODE HS00601.FORMAT HS00601.SITE HS00601.CONDITN HS00601.PAIR HS00601.DATE HS00601.TEMP HS00601.RESP_OM HS00601.RESP HS00601.DENIT_OM HS00601.DENIT HS00601.B_GLUC_OM HS00601.B_GLUC HS00601.DRYWT_OM 0 Degrees Celsius; a common unit of temperature; constantToSI=273.18 micrograms per gram per hour micromoles per g per hour micromoles per gram dimensionless number, i.e., ratio, count