uid=AND,o=lter,dc=ecoinformatics,dc=org all public read SA012 Stanley V. Gregory

Oregon State University;Dept. of Fisheries and Wildlife;Nash Hall 104 Corvallis OR 97331 USA

(541) 737-1951 stanley.gregory@oregonstate.edu http://oregonstate.edu/dept/pnw-erc/personnel/gregory.htm Judith L. Li

Oregon State University;Dept. of Fisheries & Wildlife;104 Nash Hall Corvallis OR 97331 USA

(541) 737-1093 judyli@comcast.net judith.li@oregonstate.edu http://oregonstate.edu/dept/pnw-erc/personnel/li.htm William J. Gerth

Oregon State University; Department of Fisheries and Wildlife; Nash Hall 104 Corvallis OR 97331 USA

541-737-1949 william.gerth@oregonstate.edu Other Researcher Stanley V. Gregory

Oregon State University;Dept. of Fisheries and Wildlife;Nash Hall 104 Corvallis OR 97331 USA

(541) 737-1951 stanley.gregory@oregonstate.edu http://oregonstate.edu/dept/pnw-erc/personnel/gregory.htm Creator Alan Herlihy Other Researcher Philip Kaufmann Other Researcher Judith L. Li

Oregon State University;Dept. of Fisheries & Wildlife;104 Nash Hall Corvallis OR 97331 USA

(541) 737-1093 judyli@comcast.net judith.li@oregonstate.edu http://oregonstate.edu/dept/pnw-erc/personnel/li.htm Principal Investigator Judith L. Li

Oregon State University;Dept. of Fisheries & Wildlife;104 Nash Hall Corvallis OR 97331 USA

(541) 737-1093 judyli@comcast.net judith.li@oregonstate.edu http://oregonstate.edu/dept/pnw-erc/personnel/li.htm Creator Suzanne M. Remillard

Office: FSL 354;; Forest Science Department;; Oregon State University Corvallis OR 97331 USA

(541) 758-8767 suzanne.remillard@oregonstate.edu http://orcid.org/0000-0002-0365-8866 Abstractor 2014-01-02 Total taxa richness, proportion of Ephemeroptera, Plecoptera, and Trichoptera, Shannon diversity, alpha diversity, proportion represented by the most dominant taxon, and total abundance of individuals where macroinvertebrate assemblage measures used to evaluate the effects of sampling replication and distribution in two sites (lookout and Mack Creeks) in the HJ Andrews Experimental Forest. These collections were part of a larger study that sampled 16 western Oregon streams. populations trophic structure spatial variability streams insects LTER controlled vocabulary Long-Term Ecological Research (LTER) Andrews Experimental Forest site thesaurus populations LTER core research areas Almost all assemblages were characterized by a log series abundance distribution . The number of new taxa added with increasing sample effort increased rapidly with the first 4-8 surber samples (500-1000 individuals counted), then increased more gradually. The number of new taxa added never reached zero even after counting more than 50 surber samples. Thus any measure of stream taxa richness is highly dependent on sampling effort. However, 6-9 samples captured a reasonable approximation of assemblage structure. Bootstrap analysis suggested between 65-87% of total taxa richness were described with nine samples. Stability in proportional measure values depend on the degree of dominance by one taxon, and generally stabilize between 5-10 samples in moderately diverse assemblages. A spatial analysis of variance showed differences between streams for all 5 metrics, indicating that within stream variability was not so large as to prevent detecting among stream differences. Within a stream, there was more variability among transects than within transects; this was also true for fast or slow water habitats analyzed separately. Overall, we caution against the use of protocols that sample less than 6 samples for assessing macroinvertebrate assemblages because of the excessive variability among small sample sizes and loss in assemblage structure information. We recommend a protocol of 6-9 samples spread out longitudinally along the sample stream. Related PublicationsLi, Judith; Herlihy, Alan; Gerth, William; Kaufmann, Philip; Gregory, Stanley; Urquhart, Scott; Larsen, David P. 2001. Variability in stream macroinvertebrates at multiple spatial scales. Freshwater Biology 46: 87–97. Data Use Agreement: The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. This Data Set is released under the Creative Commons license CC BY "Attribution" (see: https://creativecommons.org/licenses/by/4.0/). Creative Commons license CC BY - Attribution is a license that allows others to distribute, remix, tweak, and build upon your work (even commercially), as long as you are credited for the original creation. This license accommodates maximum dissemination and use of licensed materials. It is considered professional conduct and an ethical obligation to acknowledge the work of other scientists. The Data User is asked to provide attribution of the original work if this data package is shared in whole or by individual parts or used in the derivation of other products. A recommended citation is provided for each Data Set in the Andrews LTER data catalog (see: http://andlter.forestry.oregonstate.edu/data/catalog/datacatalog.aspx). A generic citation is also provided for this Data Set on the website https://portal.edirepository.org in the summary metadata page. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed and all data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. General acknowledgement: Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. Creative Commons Attribution 4.0 International Public License https://creativecommons.org/licenses/by/4.0/ CC-BY-4.0 https://andlter.forestry.oregonstate.edu/data/abstract.aspx?dbcode=SA012 Mack Creek and Lookout Creek, Central Western Cascades of Oregon -122.25879300 -122.09952700 44.28226000 44.20127400 422 1627 meter 1992-09-21 1992-09-30 All Organisms All Organisms Highest common category (ca. kingdom) Animalia Animal 202423 Division or Phylum Platyhelminthes flatworms 53963 Division or Phylum Annelida annelids 64357 Division or Phylum Nematoda nematodes 59490 Division or Phylum Arthropoda Arthropods 82696 Class Maxillopoda 621145 Class Insecta 99208 Order Plecoptera stoneflies 102467 Family Pteronarcyidae 102470 Family Perlodidae perlodid stoneflies 102994 Genus Skwala 103102 Genus Megarcys 103110 Genus Isoperla 102995 Genus Frisonia 103171 Genus Perlinodes 103134 Family Peltoperlidae 102488 Genus Soliperla 103142 Genus Yoraperla 102510 Family Chloroperlidae green stoneflies 103202 Genus Sweltsa 103273 Family Pteronarcidae Genus Pteronarcys 102471 Family Nemouridae 102517 Subfamily Amphinemurinae 609851 Genus Zapada 102591 Genus Soyedina 102556 Genus Malenka 102567 Genus Visoka 102614 Family Leuctridae 102840 Genus Moselia 102909 Genus Despaxia 102841 Family Perlidae common stoneflies 102914 Genus Doroneuria 103121 Genus Calineuria 102985 Genus Hesperoperla 102971 Family Capniidae 102643 Genus Eucapnopsis 102785 Genus Capnia 102688 Family Perlodidae perlodid stoneflies 102994 Genus Skwala 103102 Genus Megarcys 103110 Genus Isoperla 102995 Genus Frisonia 103171 Genus Perlinodes 103134 Family Peltoperlidae 102488 Genus Soliperla 103142 Genus Yoraperla 102510 Family Chloroperlidae green stoneflies 103202 Genus Sweltsa 103273 Family Capniidae 102643 Genus Eucapnopsis 102785 Genus Capnia 102688 Family Leuctridae 102840 Genus Moselia 102909 Genus Despaxia 102841 Family Nemouridae 102517 Subfamily Amphinemurinae 609851 Genus Zapada 102591 Genus Soyedina 102556 Genus Malenka 102567 Genus Visoka 102614 Family Perlidae common stoneflies 102914 Genus Doroneuria 103121 Genus Calineuria 102985 Genus Hesperoperla 102971 Order Megaloptera 115000 Family Sialidae 115001 Genus Sialis 115002 Family Corydalidae 115023 Genus Orohermes 115044 Family Corydalidae 115023 Genus Orohermes 115044 Family Sialidae 115001 Genus Sialis 115002 Order Diptera 118831 Family Pelecorhynchidae 130914 Genus Glutops 130915 Family Thaumaleidae 126624 Family Psychodidae 125351 Genus Pericoma 125514 Genus Maruina 125392 Family Ephydridae 146893 Family Dixidae 125809 Genus Dixa 125810 Genus Meringodixa 125873 Genus Ceratopogon 127564 Family Ceratopogonidae 127076 Genus Forcipomyiinae 127112 Genus Culicoidinae Family Empididae 135830 Genus Oreogeton 136377 Genus Clinocera 135849 Genus Chelifera 136305 Family Tipulidae 118840 Genus Cryptolabis 120488 Genus Hexatoma 120094 Genus Dicranota 121027 Genus Antocha 119656 Genus Limonia 119704 Family Tabanidae 130934 Genus Chrysops 131078 Family Dixidae 125809 Genus Dixa 125810 Genus Meringodixa 125873 Family Psychodidae 125351 Genus Pericoma 125514 Genus Maruina 125392 Family Simuliidae 126640 Genus Prosimulium 126703 Genus Simulium 126774 Family Tabanidae 130934 Genus Chrysops 131078 Family Chironomidae 127917 Genus Tanytarsini 129872 Subfamily Orthocladiinae 128457 Genus Chironomini 129229 Genus Tanypodinae 127994 Family Empididae 135830 Genus Oreogeton 136377 Genus Clinocera 135849 Genus Chelifera 136305 Family Tipulidae 118840 Genus Cryptolabis 120488 Genus Hexatoma 120094 Genus Dicranota 121027 Genus Antocha 119656 Genus Limonia 119704 Family Simulidae Order Trichoptera caddisflies 115095 Family Rhyacophilidae 115096 Genus Rhyacophila 115097 Family Polycentropodidae 117043 Genus Polycentropus 117044 Family Limnephilidae 115933 Genus Psychoglypha 115974 Genus Neophylax 116046 Genus Ecclisomyia 116025 Genus Apatania 115935 Genus Dicosmoecus 116265 Family Lepidostomatidae 116793 Genus Lepidostoma 116794 Family Hydropsychidae 115398 Genus Hydropsyche 115453 Family Calamoceratidae 116529 Genus Heteroplectron 116537 Family Philopotamidae 115257 Genus Wormaldia 115258 Family Rhyacophilidae 115096 Genus Rhyacophila 115097 Family Polycentropodidae 117043 Genus Polycentropus 117044 Family Lepidostomatidae 116793 Genus Lepidostoma 116794 Family Hydroptilidae 115629 Genus Hydroptila 115641 Genus Ochrotrichia 115714 Family Calamoceratidae 116529 Genus Heteroplectron 116537 Family Limnephilidae 115933 Genus Psychoglypha 115974 Genus Neophylax 116046 Genus Ecclisomyia 116025 Genus Apatania 115935 Genus Dicosmoecus 116265 Family Glossosomatidae 117120 Genus Glossosoma 117159 Genus Agapetus 117121 Family Brachycentridae 116905 Genus Brachycentrus 116906 Genus Micrasema 116958 Family Hydropsychidae 115398 Genus Hydropsyche 115453 Family Leptoceridae 116547 Genus Mystacides 116598 Family Psychomyiidae 115334 Genus Psychomyia 115335 Family Brachycentridae 116905 Genus Brachycentrus 116906 Genus Micrasema 116958 Family Glossosomatidae 117120 Genus Glossosoma 117159 Genus Agapetus 117121 Family Hydroptilidae 115629 Genus Hydroptila 115641 Genus Ochrotrichia 115714 Family Leptoceridae 116547 Genus Mystacides 116598 Family Philopotamidae 115257 Genus Wormaldia 115258 Family Psychomyiidae 115334 Genus Psychomyia 115335 Order Odonata 101593 Family Gomphidae 101664 Genus Octogomphus 101736 Family Gomphidae 101664 Genus Octogomphus 101736 Order Plecoptera stoneflies 102467 Family Pteronarcyidae 102470 Family Perlodidae perlodid stoneflies 102994 Genus Skwala 103102 Genus Megarcys 103110 Genus Isoperla 102995 Genus Frisonia 103171 Genus Perlinodes 103134 Family Peltoperlidae 102488 Genus Soliperla 103142 Genus Yoraperla 102510 Family Chloroperlidae green stoneflies 103202 Genus Sweltsa 103273 Family Pteronarcidae Genus Pteronarcys 102471 Family Nemouridae 102517 Subfamily Amphinemurinae 609851 Genus Zapada 102591 Genus Soyedina 102556 Genus Malenka 102567 Genus Visoka 102614 Family Leuctridae 102840 Genus Moselia 102909 Genus Despaxia 102841 Family Perlidae common stoneflies 102914 Genus Doroneuria 103121 Genus Calineuria 102985 Genus Hesperoperla 102971 Family Capniidae 102643 Genus Eucapnopsis 102785 Genus Capnia 102688 Family Perlodidae perlodid stoneflies 102994 Genus Skwala 103102 Genus Megarcys 103110 Genus Isoperla 102995 Genus Frisonia 103171 Genus Perlinodes 103134 Family Peltoperlidae 102488 Genus Soliperla 103142 Genus Yoraperla 102510 Family Chloroperlidae green stoneflies 103202 Genus Sweltsa 103273 Family Capniidae 102643 Genus Eucapnopsis 102785 Genus Capnia 102688 Family Leuctridae 102840 Genus Moselia 102909 Genus Despaxia 102841 Family Nemouridae 102517 Subfamily Amphinemurinae 609851 Genus Zapada 102591 Genus Soyedina 102556 Genus Malenka 102567 Genus Visoka 102614 Family Perlidae common stoneflies 102914 Genus Doroneuria 103121 Genus Calineuria 102985 Genus Hesperoperla 102971 Order Trichoptera caddisflies 115095 Family Rhyacophilidae 115096 Genus Rhyacophila 115097 Family Polycentropodidae 117043 Genus Polycentropus 117044 Family Limnephilidae 115933 Genus Psychoglypha 115974 Genus Neophylax 116046 Genus Ecclisomyia 116025 Genus Apatania 115935 Genus Dicosmoecus 116265 Family Lepidostomatidae 116793 Genus Lepidostoma 116794 Family Hydropsychidae 115398 Genus Hydropsyche 115453 Family Calamoceratidae 116529 Genus Heteroplectron 116537 Family Philopotamidae 115257 Genus Wormaldia 115258 Family Rhyacophilidae 115096 Genus Rhyacophila 115097 Family Polycentropodidae 117043 Genus Polycentropus 117044 Family Lepidostomatidae 116793 Genus Lepidostoma 116794 Family Hydroptilidae 115629 Genus Hydroptila 115641 Genus Ochrotrichia 115714 Family Calamoceratidae 116529 Genus Heteroplectron 116537 Family Limnephilidae 115933 Genus Psychoglypha 115974 Genus Neophylax 116046 Genus Ecclisomyia 116025 Genus Apatania 115935 Genus Dicosmoecus 116265 Family Glossosomatidae 117120 Genus Glossosoma 117159 Genus Agapetus 117121 Family Brachycentridae 116905 Genus Brachycentrus 116906 Genus Micrasema 116958 Family Hydropsychidae 115398 Genus Hydropsyche 115453 Family Leptoceridae 116547 Genus Mystacides 116598 Family Psychomyiidae 115334 Genus Psychomyia 115335 Family Brachycentridae 116905 Genus Brachycentrus 116906 Genus Micrasema 116958 Family Glossosomatidae 117120 Genus Glossosoma 117159 Genus Agapetus 117121 Family Hydroptilidae 115629 Genus Hydroptila 115641 Genus Ochrotrichia 115714 Family Leptoceridae 116547 Genus Mystacides 116598 Family Philopotamidae 115257 Genus Wormaldia 115258 Family Psychomyiidae 115334 Genus Psychomyia 115335 Order Coleoptera 109216 Family Hydraenidae minute moss beetles 112756 Family Elmidae 114093 Genus Heterlimnius 114167 Genus Ordobrevia 114235 Genus Narpus 114142 Genus Lara 114137 Genus Ampumixis 114196 Genus Optioservus 114177 Genus Zaitzevia 114205 Family Hydroscaphidae 112744 Genus Hydroscapha 112745 Family Amphizoidae 111952 Genus Amphizoa 111953 Family Psephenidae 114069 Genus Acneus 114082 Family Elmidae 114093 Genus Heterlimnius 114167 Genus Ordobrevia 114235 Genus Narpus 114142 Genus Lara 114137 Genus Ampumixis 114196 Genus Optioservus 114177 Genus Zaitzevia 114205 Family Amphizoidae 111952 Genus Amphizoa 111953 Family Dytiscidae 111963 Family Psephenidae 114069 Genus Acneus 114082 Family Staphylinidae 113265 Order Ephemeroptera mayflies 100502 Family Siphlonuridae 100951 Family Leptophlebiidae 101095 Genus Paraleptophlebia 101187 Family Baetidae 100755 Genus Baetis 100800 Genus Pseudocleon Family Ephemerellidae 101232 Genus Caudatella 101347 Genus Attenella 101338 Genus Drunella 101365 Genus Serratella 101395 Family Leptophlebiidae 101095 Genus Paraleptophlebia 101187 Family Heptageniidae 100504 Genus Cinygma 100598 Genus Cinygmula 100557 Genus Epeorus 100626 Genus Ironodes 100666 Genus Rhithrogena 100572 Family Baetidae 100755 Genus Baetis 100800 Genus Pseudocleon Family Ameletidae 568544 Genus Ameletus 100996 Family Ephemerellidae 101232 Genus Caudatella 101347 Genus Attenella 101338 Genus Drunella 101365 Genus Serratella 101395 Family Heptageniidae 100504 Genus Cinygma 100598 Genus Cinygmula 100557 Genus Epeorus 100626 Genus Ironodes 100666 Genus Rhithrogena 100572 Family Heptgeniidae Genus Heptagenia 100602 Order Diptera 118831 Family Pelecorhynchidae 130914 Genus Glutops 130915 Family Thaumaleidae 126624 Family Psychodidae 125351 Genus Pericoma 125514 Genus Maruina 125392 Family Ephydridae 146893 Family Dixidae 125809 Genus Dixa 125810 Genus Meringodixa 125873 Genus Ceratopogon 127564 Family Ceratopogonidae 127076 Genus Forcipomyiinae 127112 Genus Culicoidinae Family Empididae 135830 Genus Oreogeton 136377 Genus Clinocera 135849 Genus Chelifera 136305 Family Tipulidae 118840 Genus Cryptolabis 120488 Genus Hexatoma 120094 Genus Dicranota 121027 Genus Antocha 119656 Genus Limonia 119704 Family Tabanidae 130934 Genus Chrysops 131078 Family Dixidae 125809 Genus Dixa 125810 Genus Meringodixa 125873 Family Psychodidae 125351 Genus Pericoma 125514 Genus Maruina 125392 Family Simuliidae 126640 Genus Prosimulium 126703 Genus Simulium 126774 Family Tabanidae 130934 Genus Chrysops 131078 Family Chironomidae 127917 Genus Tanytarsini 129872 Subfamily Orthocladiinae 128457 Genus Chironomini 129229 Genus Tanypodinae 127994 Family Empididae 135830 Genus Oreogeton 136377 Genus Clinocera 135849 Genus Chelifera 136305 Family Tipulidae 118840 Genus Cryptolabis 120488 Genus Hexatoma 120094 Genus Dicranota 121027 Genus Antocha 119656 Genus Limonia 119704 Family Simulidae Order Coleoptera 109216 Family Hydraenidae minute moss beetles 112756 Family Elmidae 114093 Genus Heterlimnius 114167 Genus Ordobrevia 114235 Genus Narpus 114142 Genus Lara 114137 Genus Ampumixis 114196 Genus Optioservus 114177 Genus Zaitzevia 114205 Family Hydroscaphidae 112744 Genus Hydroscapha 112745 Family Amphizoidae 111952 Genus Amphizoa 111953 Family Psephenidae 114069 Genus Acneus 114082 Family Elmidae 114093 Genus Heterlimnius 114167 Genus Ordobrevia 114235 Genus Narpus 114142 Genus Lara 114137 Genus Ampumixis 114196 Genus Optioservus 114177 Genus Zaitzevia 114205 Family Amphizoidae 111952 Genus Amphizoa 111953 Family Dytiscidae 111963 Family Psephenidae 114069 Genus Acneus 114082 Family Staphylinidae 113265 Order Megaloptera 115000 Family Sialidae 115001 Genus Sialis 115002 Family Corydalidae 115023 Genus Orohermes 115044 Family Corydalidae 115023 Genus Orohermes 115044 Family Sialidae 115001 Genus Sialis 115002 Order Ephemeroptera mayflies 100502 Family Siphlonuridae 100951 Family Leptophlebiidae 101095 Genus Paraleptophlebia 101187 Family Baetidae 100755 Genus Baetis 100800 Genus Pseudocleon Family Ephemerellidae 101232 Genus Caudatella 101347 Genus Attenella 101338 Genus Drunella 101365 Genus Serratella 101395 Family Leptophlebiidae 101095 Genus Paraleptophlebia 101187 Family Heptageniidae 100504 Genus Cinygma 100598 Genus Cinygmula 100557 Genus Epeorus 100626 Genus Ironodes 100666 Genus Rhithrogena 100572 Family Baetidae 100755 Genus Baetis 100800 Genus Pseudocleon Family Ameletidae 568544 Genus Ameletus 100996 Family Ephemerellidae 101232 Genus Caudatella 101347 Genus Attenella 101338 Genus Drunella 101365 Genus Serratella 101395 Family Heptageniidae 100504 Genus Cinygma 100598 Genus Cinygmula 100557 Genus Epeorus 100626 Genus Ironodes 100666 Genus Rhithrogena 100572 Family Heptgeniidae Genus Heptagenia 100602 Order Odonata 101593 Family Gomphidae 101664 Genus Octogomphus 101736 Family Gomphidae 101664 Genus Octogomphus 101736 Class Oligochaeta 68422 Class Gastropoda 69459 Order Prosobranchia 78780 Family Pleuroceridae 71541 Genus Juga 71570 Family Hydrobiidae 70493 Class Collembola 99237 Order Collembola 99237 Class Arachnida 82708 Subclass Acari 733321 Order Hydracarina Class Turbellaria 53964 Class Ostracoda 84195 Subphylum Crustacea 83677 Class Malacostraca 89787 Order Decapoda 95599 Family Astacidae crayfishes 97324 Genus Pacifastacus 97325 Order Harpacticoida 86110 Division or Phylum Mollusca mollusks 69458 Class Pelecypoda bivalves 79119 Order Corbiculaceae Family Sphaeriidae 81389 Division or Phylum Mollusca mollusks 69458 Class Pelecypoda bivalves 79119 Order Corbiculaceae Family Sphaeriidae 81389

An update history is logged and maintained with each new version of every dataset.

notPlanned Original metadata creation. Version1 1995-10-20 Metadata restructured and moved into SQLServer metadata database LTERMETA. Data moved into SQLServer database FSDBDATA. Version2 2001-07-04 Converted integers to numeric for better conversion. Added place_keyword and enumerated STCODE. Modified some of the study level metadata to represent the data and not the broader study for which no data are present. Linked study to the broader study publication. Enumerated all taxonomies. Recreate CSV files for uploading into PASTA. Version7 2014-01-02 Information Manager

Andrews Forest LTER Program US Forest Service Pacific Northwest Research Station 3200 SW Jefferson Way Corvallis OR 97331

hjaweb@lists.oregonstate.edu http://andrewsforest.oregonstate.edu/ Donald L. Henshaw

Pacific Northwest Research Station; Corvallis Forestry Sciences Lab; 3200 SW Jefferson Way Corvallis Oregon 97331

hja_admin@lists.oregonstate.edu http://orcid.org/0000-0001-5952-4528 Andrews Forest LTER Site

Forest Ecosystems and Society Department in Forestry Oregon State University 201K Richardson Hall Corvallis OR 97331-5752

(541) 737-8480 lterweb@lists.oregonstate.edu http://andrewsforest.oregonstate.edu/ Field Methods - SA012 Site Selection : Two hand-picked stream sites (Lookout and Mack Creeks) in the H.J. Andrews Experimental Forest/Cascade Mt. Long-term Ecological Research area were also sampled to allow comparison to the large available database at these sites. Mack Creek is a small first order stream, Lookout Creek is a fairly large third order stream. The collection at these streams were added to 16 streams (9 Cascade and 7 Valley) that were sampled for macroinvertebrate assemblages as part of a larger study. Field Sampling and Counting: Macroinvertebrate field sampling took place in late September, 1992. From the random start point on each stream, 14 study transects (cross-sections) were marked off upstream at 10 m intervals in small streams, and 25 m intervals in large streams. Three, one ft2 Surber samples, one on the right side, center and left were taken within each transect (Fig. 2). At least 7 transects of fast water habitat (riffles, rapids or cascades) and 7 transects of slow-water habitat (pools or glides) were sampled on each stream. If 7 of each habitat were not encountered after 14 transects, the crew continued upstream sampling at the same transect intervals in only the under-represented habitat until 7 were sampled. As a result, total number of transects varied from 14 to 20 per stream. Each macroinvertebrate sample was filtered through a 500 micro-meter soil sieve at stream side. Samples were placed in whirlbags and preserved in 70% ethanol. Stream habitat at each Surber sample location was qualitatively classified as either a pool, glide, riffle, rapid, or cascade. Laboratory Methods - SA012 Invertebrate samples were sorted and counted in the laboratory. At least 100 organisms, or all organisms, whichever count was achieved first, were enumerated for all samples. In 378 of the 449 samples, the entire sample was counted. If organisms were extremely dense or organic debris made enumeration very difficult the original sample was subsampled using a 0.5 m subsampler grid (Caton et al 1991). If subsampling was necessary, final counts were calculated by multiplying the observed count by the inverse of the proportion counted. Insect taxa,with the exception of Chironomidae, were identified to genus; Chironomidae were identified to tribe. Molluscs, Branchiopoda, and Copepoda were identified to family. Other invertebrate groups were identified to order. These taxa groupings were used in calculating metrics including taxa richness, and non-insectan taxa. Sampling frequency: once The diversity and environmental sensitivity of lotic invertebrates make them useful indicators of stream conditions; consequently macroinvertebrates have become increasingly popular in regional comparisons of streams (Hilsehoff 1988; Kerans and Kerr 1994; Rosenberg and Resh 1993). However, spatial and temporal variability in abundance and diversity among individual collections (at the reach scale) can make detection of differences among streams difficult (Norris and Georges 1993) necessitating some level of replication or physical compositing of samples. A dilemma we often face is how to determine the adequate number of samples. One approach has been to adjust the number of replicates to yield the desired level of precision (Elliott, 1977; Resh 1979); a criterion of ñ40% mean total abundances has been suggested as a practical goal (Resh and McElravy 1993; Pinder el al 1987). Another approach has been to evaluate the stability of the mean with increasing sampling effort (Elliott, 1977). These methods are limited by relying heavily on numerical abundance, with little regard for other assemblage attributes. Our approach for assessing sampling adequacy was to consider a number of broadly representative metrics, and to identify appropriate levels of sampling effort to achieve stable values of these assemblage measures, somewhat analogous to one method suggested in Elliott (1977). Biomonitoring programs often use descriptors (often called metrics) designed to represent three basic assemblage characteristics: numerical abundance (total numbers of individuals), measures of diversity (taxa richness, diversity indices, and measures of evenness or dominance), and proportional abundances (proportions of total numbers including groups or taxa of special interest such as taxonomic order, dominant taxa, functional feeding groups or pollution-tolerant taxa). Some of these measures overlap, particularly those based on proportions that are all dependent on the same denominator, e.g. total abundance. We chose five metrics that represent different aspects of assemblage components: total abundance, taxa richness, Shannon diversity, proportional relative abundance of Ephemeroptera, Trichoptera and Plecoptera (EPT), and percent dominance of the most dominant taxon. In the article associated with this study, our focus was on the adequacy of field macroinvertebrate sampling to discern differences among stream reaches within ecoregions. We greatly oversampled each stream reach, examining the incremental changes in metric values resulting from increases in sampling effort within each stream reach. This intense, hierarchical sampling design allowed us to quantitatively compare the relative magnitudes of macroinvertebrate assemblage metric variances across the range of spatial scales from within-habitat (eg. within a riffle) up to within-region (ie, comparing ecoregions). Andrews Experimental Forest (HJA) -122.26172200 -122.10084700 44.28196400 44.19770400 1631 1631 meter Mack Creek -122.16826200 -122.14698900 44.22714600 44.20991900 Andrews Lookout Creek Watershed -122.25879300 -122.09952700 44.28226000 44.20127400 1627 1627 meter Andrews Mack Creek Watershed -122.17282200 -122.12699600 44.22054300 44.20148000 1626 1626 meter Posy Elizabeth Busby

Assistant Professor OSU Botany & Plant Pathology

busbyp@science.oregonstate.edu posybusby@gmail.com http://bpp.oregonstate.edu/content/posy-busby http://orcid.org/0000-0002-2837-9820 Principal Investigator Matthew G Betts

Department of Forest Ecosystems and Society; 201E Richardson Hall; College of Forestry; Oregon State University Corvallis OR 97331

(541) 737-3841 matt.betts@oregonstate.edu http://www.fsl.orst.edu/flel/index.htm http://orcid.org/0000-0002-7100-2551 Principal Investigator Brooke E. Penaluna brooke.penaluna@usda.gov Brooke.Penaluna@oregonstate.edu https://www.fs.fed.us/pnw/lwm/aem/people/penaluna.html http://orcid.org/0000-0001-7215-770X Principal Investigator Catalina Segura

Assistant Professor; Department of Forest Engineering, Resources, and Management; Oregon State University Corvallis OR 97331

541-737-6568 catalina.segura@oregonstate.edu http://ferm.forestry.oregonstate.edu/facstaff/segura-catalina http://orcid.org/0000-0002-0924-1172 Principal Investigator David Bell david.bell@usda.gov david.bell@oregonstate.edu https://lemma.forestry.oregonstate.edu/about/david-bell http://orcid.org/0000-0002-2673-5836 Principal Investigator The H.J. Andrews Experimental Forest is a living laboratory that provides unparalleled opportunities for the study of forest and stream ecosystems in the central Cascade Range of Oregon. Since 1980, as a part of the National Science Foundation Long Term Ecological Research (NSF-LTER) program, the Andrews Experimental Forest has become a leader in the analysis of forest and stream ecosystem dynamics. Long-term field experiments and measurement programs have focused on climate dynamics, streamflow, water quality, and vegetation succession. Currently researchers are working to develop concepts and tools needed to predict effects of natural disturbance, land use, and climate change on ecosystem structure, function, and species composition. The Andrews Experimental Forest is administered cooperatively by the USDA Forest Service Pacific Northwest Research Station, Oregon State University and the Willamette National Forest. Funding for the research program comes from the National Science Foundation (NSF), US Forest Service Pacific Northwest Research Station, Oregon State University, and other sources. Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. National Science Foundation: DEB2025755 National Science Foundation https://ror.org/021nxhr62 DEB2025755 https://www.nsf.gov/awardsearch/showAward?AWD_ID=2025755 The Andrews Forest is situated in the western Cascade Range of Oregon, and covers the entire 15,800-acre (6400-ha) drainage basin of Lookout Creek. Elevation ranges from 1350 to 5340 feet (410 to 1630 m). Broadly representative of the rugged mountainous landscape of the Pacific Northwest, the Andrews Forest contains excellent examples of the region's conifer forests and associated wildlife and stream ecosystems. These forests are among the tallest and most productive in the world, with tree heights of often greater than 250 ft (75 m). Streams are steep, cold and clean, providing habitat for numerous aquatic organisms. SA01201 Macroinvertebrate Species of the Andrews Forest SA01201_v2.csv 23359 556753f4ca5091fa8189d5b8665d6e27 1 \r\n column , " https://andlter.forestry.oregonstate.edu/data/register/dataaccess.aspx?docid=SA01201_v2.csv 1992-06-01 1993-09-30 STCODE FSDB Database Study code char(5) SA012 FSDB Database code SA012 FORMAT Format number numeric(2,0) number 1.000000 natural 1.0000 1.0000 LOCATION Location of species varchar(15) Mack Creek Andrews Mack Creek Watershed Lookout Creek Andrews Lookout Creek Watershed YEAR Year sampled numeric(4,0) number 1.000000 PHYLUM Taxonomic category: phylum varchar(17) Arthropoda Arthropoda Nematoda Nematoda Annelida Annelida Mollusca Mollusca Platyhelminthes Platyhelminthes CLASS Taxonomic category: class varchar(15) Acari Acari Turbellaria Turbellaria Gastropoda Gastropoda Oligochaeta Oligochaeta Ostracoda Ostracoda Insecta Insecta Malacostraca Malacostraca Maxillopoda Maxillopoda Pelecypoda Pelecypoda TAX_ORDER Taxonomic category: order varchar(16) Hydracarina Hydracarina Collembola Collembola Prosobranchia Prosobranchia Coleoptera Coleoptera Trichoptera Trichoptera Corbiculaceae Corbiculaceae Diptera Diptera Ephemeroptera Ephemeroptera Megaloptera Megaloptera Odonata Odonata Plecoptera Plecoptera Decapoda Decapoda Harpacticoida Harpacticoida FAMILY Taxonomic category: family varchar(22) Pleuroceridae Pleuroceridae Hydroscaphidae Hydroscaphidae Sphaeriidae Sphaeriidae Tabanidae Tabanidae Ephemerellidae Ephemerellidae Amphizoidae Amphizoidae Baetidae Baetidae Brachycentridae Brachycentridae Calamoceratidae Calamoceratidae Capniidae Capniidae Ceratopogonidae Ceratopogonidae Chironomidae Chironomidae Chloroperlidae Chloroperlidae Corydalidae Corydalidae Dixidae Dixidae Dytiscidae Dytiscidae Elmidae Elmidae Empididae Empididae Ephydridae Ephydridae Glossosomatidae Glossosomatidae Gomphidae Gomphidae Heptageniidae Heptageniidae Hydropsychidae Hydropsychidae Hydroptilidae Hydroptilidae Lepidostomatidae Lepidostomatidae Leptoceridae Leptoceridae Leptophlebiidae Leptophlebiidae Leuctridae Leuctridae Limnephilidae Limnephilidae Nemouridae Nemouridae Peltoperlidae Peltoperlidae Perlidae Perlidae Perlodidae Perlodidae Philopotamidae Philopotamidae Polycentropodidae Polycentropodidae Psephenidae Psephenidae Psychodidae Psychodidae Psychomyiidae Psychomyiidae Rhyacophilidae Rhyacophilidae Sialidae Sialidae Siphlonuridae Siphlonuridae Staphylinidae Staphylinidae Thaumaleidae Thaumaleidae Tipulidae Tipulidae Pelecorhynchidae Pelecorhynchidae Simulidae Simulidae Pteronarcyidae Pteronarcyidae Astacidae Astacidae Hydraenidae Hydraenidae Hydrobiidae Hydrobiidae GENUS Taxonomic category: genus varchar(24) Juga Juga Acneus Acneus Agapetus Agapetus Ameletus Ameletus Amphizoa Amphizoa Glossosoma/anagapetus Anagapetus Antocha Antocha Apatania Apatania Attenella Attenella Baetis Baetis Brachycentrus Brachycentrus Calineuria Calineuria Capnia Capnia Caudatella Caudatella Chrysops Chrysops Cinygma Cinygma Cinygmula Cinygmula Clinocera Clinocera Hydroscapha Hydroscapha Despaxia Despaxia Dicosmoecus Dicosmoecus Dicranota Dicranota Dixa Dixa Doroneuria Doroneuria Drunella Drunella Ecclisomyia Ecclisomyia Epeorus Epeorus Eucapnopsis Eucapnopsis Frisonia Frisonia Glossosoma/anagapetus Glossosoma Hesperoperla Hesperoperla Heteroplectron Heteroplectron Hydropsyche Hydropsyche Hydroptila Hydroptila Ironodes Ironodes Isoperla Isoperla Lara Lara Lepidostoma Lepidostoma Limonia Limonia Malenka Malenka Maruina Maruina Megarcys Megarcys Micrasema Micrasema Moselia Moselia Mystacides Mystacides Neophylax Neophylax Ochrotrichia Ochrotrichia Octogomphus Octogomphus Orohermes Orohermes Paraleptophlebia Paraleptophlebia Perlinodes Perlinodes Polycentropus Polycentropus Prosimulium Prosimulium Psychoglypha Psychoglypha Psychomyia Psychomyia Pteronarcys Pteronarcys Rhithrogena Rhithrogena Rhyacophila Rhyacophila Serratella Serratella Sialis Sialis Simulium Simulium Skwala Skwala Soliperla Soliperla Soyedina Soyedina Sweltsa Sweltsa Visoka Visoka Wormaldia Wormaldia Yoraperla Yoraperla Zapada Zapada Ampumixis Ampumixis Narpus Narpus Optioservus Optioservus Ordobrevia Ordobrevia Chelifera Chelifera Oreogeton Oreogeton Glutops Glutops Hexatoma Hexatoma Heptagenia Heptagenia Hydraena (adult) Hydraenidae Chironomini Chironomini Forcipomyiinae Forcipomyiinae Heterlimnius Heterlimnius Meringodixa Meringodixa Orthocladinae Orthocladiinae Pacifastacus Pacifastacus Pericoma/telmatoscopus Pericoma Tanypodinae Tanypodinae Tanytarsini Tanytarsini Zaitzevia Zaitzevia Pericoma/telmatoscopus Telmatoscopus Cryptolabis Cryptolabis Culicoidinae Culicoidinae Malenka/amphinemura Amphinemurinae Malenka/amphinemura Amphinemura Pseudocleon Pseudocleon NSURBERS Number of samples which contained individuals of the species numeric(2,0) number 1.000000 natural 1.0000 43.0000 ABUND Total number of individuals of the species from all samples numeric(4,0) number 1.000000 natural 1.0000 2961.0000 PRIMARY SA01201.LOCATION SA01201.YEAR NOTNULL SA01201.ABUND SA01201.FORMAT SA01201.LOCATION SA01201.NSURBERS SA01201.STCODE SA01201.YEAR 249