uid=AND,o=lter,dc=ecoinformatics,dc=org all public read SP006 Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Principal Investigator Bruce A. Caldwell

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-3674 bruce.caldwell@oregonstate.edu Other Researcher Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Abstractor Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Creator 2013-12-17 To conduct a comprehensive study of soil chemical and microbiological properties at the HJA during the week of July 11,1994. biology soil chemistry temperature air temperature respiration denitrification inorganic nutrients nitrogen microbes LTER controlled vocabulary Long-Term Ecological Research (LTER) Andrews Experimental Forest site thesaurus inorganic nutrients LTER core research areas Data Use Agreement: The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. This Data Set is released under the Creative Commons license CC BY "Attribution" (see: https://creativecommons.org/licenses/by/4.0/). Creative Commons license CC BY - Attribution is a license that allows others to distribute, remix, tweak, and build upon your work (even commercially), as long as you are credited for the original creation. This license accommodates maximum dissemination and use of licensed materials. It is considered professional conduct and an ethical obligation to acknowledge the work of other scientists. The Data User is asked to provide attribution of the original work if this data package is shared in whole or by individual parts or used in the derivation of other products. A recommended citation is provided for each Data Set in the Andrews LTER data catalog (see: http://andlter.forestry.oregonstate.edu/data/catalog/datacatalog.aspx). A generic citation is also provided for this Data Set on the website https://portal.edirepository.org in the summary metadata page. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed and all data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. General acknowledgement: Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. http://andlter.forestry.oregonstate.edu/data/abstract.aspx?dbcode=SP006 Andrews Experimental Forest -122.25800000 -122.10400000 44.00000000 44.20500000 1472.105 433.856 meter 1994-07-11 1994-07-15

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irregular Original metadata creation. Version1 1994-01-04 Metadata restructured and moved into SQLServer metadata database LTERMETA. Data moved into SQLServer database FSDBDATA. Version2 2002-04-10 Converted STCODE to enumerated. Ran QC check. Recreate csv files for new delimiter for upload into PASTA. Version7 2013-12-17 Information Manager

Andrews Forest LTER Program US Forest Service Pacific Northwest Research Station 3200 SW Jefferson Way Corvallis OR 97331

hjaweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Robert P. Griffiths

Oregon State University;Dept. of Forest Science;321 Richardson Hall Corvallis OR 97331-5752 USA

(541) 737-6559 bbgriff@peak.org griff@for.orst.edu Andrews Forest LTER Site

Forest Ecosystems and Society Department in Forestry Oregon State University 201K Richardson Hall Corvallis OR 97331-5752

(541) 737-8480 lterweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Field Methods - SP006 1. The sites were located approximately every 0.5 km along all accessible roads on the HJA. 2. During the first day, we installed in incubation chambers (3 gal plastic ice cream tubs fitted with a serum bottle septum for collection of headspace gas analyses) installed in the sodalime jars, took cores for bulk density and percent moisture and took soil and air temperature readings. All vegetation except moss was removed from the ground covered by the incubation chambers. Those chambers covering moss was noted as such. 3. On the second day, we took headspace gas samples and placed them in Vacutainers for subsequent analysis for CO2 concentrations using a gas chromatograph. The sodalime was retrieved, air and soil temperatures measured and cores were taken for subsequent chemical and biological analyses. Laboratory Methods - SP006 1. Soil Preparation Before sieving the soils, subsamples were taken for DOC and laboratory respirations. The soils were then sieved through a 2 mm sieve for subsequent analysis. 2. Respiration measurements (laboratory). Five g field-moist samples were placed in a 25 mL Erlenmeyer flask fitted with a serum bottle stopper. Three separate subsamples were run for each sample. To one, nothing was added, to another, 2 mL distilled sterile water was added and to a third 2 mL of 10-3 M sterile glucose (dextrose) solution. After the samples were preincubated for one hr at 19°C, the first headspace analyses was made. Two hours later after being incubated at the same temperature, another headspace analysis was conducted. The net increase in CO2 concentrations was calculated by subtracting the first reading from the second. The concentration of CO2 was measured on a gas chromatograph fitted with a methanizer and a flame ionization detector. The instrument was calibrated with a standard calibration gas at 0.997 %. Substrate induced respiration (SIR) was calculated by subtracting the CO2 released from the water amended soils from the CO2 released from the glucose amended soils. 3. Dissolved organic carbon (DOC) Five g of wet weight soil was diluted with 15 ml of water in a 100 mL serum bottle. The soils are then shaken for one hour at room temperature and then allowed to stand for one hour. One and one half ml of the slurry is removed and centrifuged in a microfuge for 5 minutes. After centrifuging, 0.5 ml of the supernatant was removed and placed in a 0.5 ml centrifuging tube. Two blanks containing just deionized water are also run with the set. The samples are then frozen. Before analysis the samples were thawed and vortexed to suspend the precipitate. The samples were analyzed for organic carbon using a carbon analyzer. 4. Percent moisture Two 10 g field-moist soil samples are measured into Al weighing boats and dried at 100°C for 8 h before dried weight determinations were made. Percent moisture was calculated as ((wet wt.- dry wt)/dry wt)*100. 5. Soil organic matter (SOM) One 1-2 g subsample of dried soil (100°C for 8-12 hr) was placed in a preweighed porcelain crucible and reweighed. The sample was fired to 550°C in a furnace for 6 h. After firing, the samples were weighed again. The resulting data were expressed as a percent of the weight loss relative to the initial dried wt of the sample. 6. PH Five gdw soil was added to 50 ml deionized water. The soil slurries were shaken for a least one h before pH measurements were made. The pH meter was calibrated using pH 4.0 and 7.0 standards. The samples and standards were stirred while readings were made. 7. Extractable ammonium concentrations Replicate 10 g field moist soils were added to 50 ml 2M KCl in 250 ml Erlenmeyer flasks and shaken for one h. in the presence of 0.4 ml 10M NaOH. The specific ion meter used in to make this measurements was calibrated using a set of ammonium standards made up in 2M KCl. An Orion ammonia electrode was use to make the measurements. 8. Mineralizable nitrogen Ten g of field-moist soil was added to a large screw-topped test tube and filled with deionized water, shaken with the cap on to make sure all soil was saturated. The tubes were then filled with water and sealed with no gas in the headspace. The tubes were then incubated at 40°C for 1 week. After the soils have incubated, they were placed into 250 ml flasks and an equal amount (50 ml) of 4M KCl was added. One third ml of 10 M NaCl were added to the flasks and they are then shaken on a rotary shaker for at least one h before the ammonium concentrations were measured. The ammonium concentrations were measured using the same technique used to measure extractable ammonium. The net mineralizable nitrogen was calculated by subtracting the extractable ammonium concentrations from the ammonium concentrations after 1 week incubation. 9. Denitrification potential The soils were prepared using the same procedure as that for laboratory respirations except nonseived soils were used and the headspace was purged with Ar for a minimum of 3 mins. at over 100 mL per min. The flasks were incubated for one hr at room temperature then one or two mL of a sterile solution containing 10-3 M glucose and nitrate was injected through the stopper. The soils were incubated for another hour at room temperature at which time the time 0 reading is taken. After two more hours, a second headspace analysis was made for nitrous oxide concentration using the electron capture detector. 10. Field respiration using sodalime and headspace analyses Eight oz wide mouth glass bottles with metal and plastic lids were filled with 30 g 6-12 mesh sodalime. These jars were baked at 100°C for 8 h, weighed and sealed before they were placed in the incubation chambers in the field. After they were opened over 24 h to collect the CO2, they were sealed, returned to the lab and baked again with the lids off. The jar were then reweighed that weight gain recorded. After the incubation chambers had been in place for 24 h and the CO2 adsorbed, a 6", 20 gauge needle and a 60 mL syringe was used to remove a headspace sample. One syringe- full of gas was pulled into the syringe and then expelled back into the chamber and then a second syringe-full was extracted from the center of the chamber. All but 5 mL of the 60 were used to purge a 7 mL vacutainer. The final 5 mL were used to overpressure the tubes. The gas in the Vacutainer was analyzed for CO2 using the same GC set-up used to measure laboratory respiration rates. The total CO2 production was calculated by subtracting out the controls for both soda-lime and headspace CO2 (chambers that were sealed at the beginning of the incubation period). The total CO2 released was calculated using the volume of the chambers and the area trapped under the incubation chamber. 11. Mean temperatures Soil temperatures (to 5 cm) and air temperatures were take during both days in the field using a simple dial thermometer. The mean values are the average for the two days. 12. Bulk density Bulk density measurements were made by drying 4.7 x 10 cm cores at 100°C for 8 h. 13. Site location and elevation The site locations were estimated on a map of the HJA and digitized into the GIS system. From these location, elevations were estimated using data already in the system. Sampling frequency: once only During the same 24 hour period, all 182 sites studied were sampled. These sites represent the best possible geographical coverage from accessible roads at the HJA. Andrews Experimental Forest (HJA) -122.26172200 -122.10084700 44.28196400 44.19770400 1631 1631 meter Sherri L. Johnson

US Forest Service ;Pacific NW Research Station ;3200 SW Jefferson Way Corvallis OR 97331 USA

541-758-7771 sherri.johnson2@usda.gov sherri.johnson@oregonstate.edu https://www.fs.fed.us/research/people/profile.php?alias=sherrijohnson Principal Investigator Julia A. Jones

Oregon State University;Department of Geosciences; Wilkinson Hall 104 Corvallis OR 97331-5506 USA

(541) 737-1224 Julia.Jones@oregonstate.edu geojulia@comcast.net http://ceoas.oregonstate.edu/profile/jones/ http://orcid.org/0000-0001-9429-8925 Principal Investigator Matthew G Betts

Department of Forest Ecosystems and Society; 201E Richardson Hall; College of Forestry; Oregon State University Corvallis OR 97331

(541) 737-3841 matt.betts@oregonstate.edu http://www.fsl.orst.edu/flel/index.htm Principal Investigator Michael P. Nelson

Department of Forest Ecosystems and Society; 201K Richarson Hall; College of Forestry; Oregon State University Corvallis OR 97331

541-737-9221 mpnelson@oregonstate.edu http://www.michaelpnelson.com http://orcid.org/0000-0001-6917-4752 Principal Investigator David Bell david.bell@usda.gov david.bell@oregonstate.edu https://lemma.forestry.oregonstate.edu/about/david-bell Principal Investigator The H.J. Andrews Experimental Forest is a living laboratory that provides unparalleled opportunities for the study of forest and stream ecosystems in the central Cascade Range of Oregon. Since 1980, as a part of the National Science Foundation Long Term Ecological Research (NSF-LTER) program, the Andrews Experimental Forest has become a leader in the analysis of forest and stream ecosystem dynamics. Long-term field experiments and measurement programs have focused on climate dynamics, streamflow, water quality, and vegetation succession. Currently researchers are working to develop concepts and tools needed to predict effects of natural disturbance, land use, and climate change on ecosystem structure, function, and species composition. The Andrews Experimental Forest is administered cooperatively by the USDA Forest Service Pacific Northwest Research Station, Oregon State University and the Willamette National Forest. Funding for the research program comes from the National Science Foundation (NSF), US Forest Service Pacific Northwest Research Station, Oregon State University, and other sources. Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. National Science Foundation: DEB1440409 The Andrews Forest is situated in the western Cascade Range of Oregon, and covers the entire 15,800-acre (6400-ha) drainage basin of Lookout Creek. Elevation ranges from 1350 to 5340 feet (410 to 1630 m). Broadly representative of the rugged mountainous landscape of the Pacific Northwest, the Andrews Forest contains excellent examples of the region's conifer forests and associated wildlife and stream ecosystems. These forests are among the tallest and most productive in the world, with tree heights of often greater than 250 ft (75 m). Streams are steep, cold and clean, providing habitat for numerous aquatic organisms. 1 SP00601 Site Locations and Elevations: SP00601.csv 6801 f687ea57cc2af8904d8d991fcced5498 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP00601_v2.csv 1994-07-11 1994-07-15 STCODE FSDB Database Study code char(5) SP006 FSDB Database Study Code SP006 FORMAT Entity number numeric(1,0) number 1.000000 natural 1.0000 1.0000 SITE Site name numeric(4,0) number 1.000000 natural 1.0000 202.0000 LATITUDE Latitude of site numeric(7,3) degrees latitude or longitude 0.001000 real 44.2050 44.2770 LONGITUDE Longitude of site numeric(9,3) degrees latitude or longitude 0.001000 real -122.2580 -122.1040 ELEVATION Elevation of site numeric(9,3) meters 0.001000 real 433.8560 1472.1050 PRIMARY SP00601.SITE NOTNULL SP00601.ELEVATION SP00601.FORMAT SP00601.LATITUDE SP00601.LONGITUDE SP00601.SITE SP00601.STCODE 184 2 SP00602 Soil Chemical and Microbiological Properties: SP00602.csv 24534 599e8ae783f542c989e36f2514ae9e59 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP00602_v2.csv 1994-07-11 1994-07-15 STCODE FSDB Database Study code char(5) SP006 FSDB Database Study Code SP006 FORMAT Entity number numeric(1,0) number 1.000000 natural 2.0000 2.0000 SITE Site name numeric(4,0) number 1.000000 natural 1.0000 202.0000 MOS Moss presence char(2) 0 No moss present 1 Some moss present 2 Mostly moss MATS Mat presence char(2) 0 Mats not present 1 Mats are present STANDAGE Stand successional stage char(2) 1 Oldgrowth 2 Mature 3 Yount 4 Green-tree 5 Clearcut PH Soil pH numeric(6,2) pH units 0.010000 real 3.1000 6.0000 PERMOIST Percent moisture numeric(7,2) percent 0.010000 real 9.6500 177.7800 AIRTEMP Mean air temperature for two days numeric(6,2) degrees Celsius 0.010000 real 12.9200 34.7200 SOILTEMP Mean soil temperature for two days numeric(6,2) degrees Celsius 0.010000 real 5.8500 27.7800 BULKDENS Bulk density numeric(5,2) grams per milliliter 0.010000 real 0.1000 1.1800 SOM Soil organic matter numeric(6,2) percent 0.010000 real 7.3700 90.6300 WEEK7RSP Seven week accumulated CO2 (long-term respiration) numeric(8,2) number 0.010000 real 7.5000 1782.5000 RESP Field respiration by sodalime (as C) numeric(5,2) grams per square meter per day 0.010000 real 0.1800 4.5800 HEADSPAC Headspace carbon (as C) numeric(6,3) grams per square meter 0.001000 real 0.0020 0.0330 TOTALRES Total field respiration (as c) numeric(5,2) grams per square meter per day 0.010000 real 0.1800 4.6000 LABRESP Laboratory respiration (no amendments, as C) numeric(5,2) micrograms per gram per hour 0.010000 real 0.0000 1.8300 H2ORESP Laboratory respiration (water added, as C) numeric(6,2) micrograms per gram per hour 0.010000 real -0.0600 2.1000 GLUCRESP Laboratory respiration (glucose in water, as C) numeric(5,2) micrograms per gram per hour 0.010000 real 0.0000 1.9000 SIR Substrate induced respiration (as C) numeric(6,2) micrograms per gram per hour 0.010000 real -0.2000 0.6700 DENITR Denitrification potential (as C) numeric(7,2) micrograms per gram per hour 0.010000 real 0.0000 518.0300 EXTR_AMM Extractable ammonium (as N, dry weight basis)) numeric(6,2) micrograms per gram 0.010000 real 0.0000 24.1600 NETMIN_N Net mineralizable nitrogen (as N, dry weight basis)) numeric(7,2) micrograms per gram 0.010000 real 5.3100 221.9300 LAB_SOM Labresp value / SOM numeric(7,4) number 0.000100 real 0.0000 0.0520 GLUC_SOM Glucose enhanced respiration / SOM numeric(7,4) number 0.000100 real 0.0000 0.0674 LONG_MIN Week7rsp value / mineralizable nitrogen numeric(6,2) number 0.010000 real 0.2100 38.9200 LONG_SOM Week7rsp value / som numeric(6,2) number 0.010000 real 0.4700 23.0400 PRIMARY SP00602.SITE NOTNULL SP00602.AIRTEMP SP00602.DENITR SP00602.FORMAT SP00602.GLUCRESP SP00602.H2ORESP SP00602.LABRESP SP00602.MATS SP00602.PERMOIST SP00602.PH SP00602.SIR SP00602.SITE SP00602.SOILTEMP SP00602.STCODE 183 meter; SI unit of length degrees; latitude (parallel) or longitude (meridian) grams per milliliter grams per square meter micrograms per gram Scale used for pH measurements micrograms per gram per hour Degrees Celsius; a common unit of temperature; constantToSI=273.18 percent; a number grams per square meter per day dimensionless number, i.e., ratio, count