uid=AND,o=lter,dc=ecoinformatics,dc=org all public read SP033 Barbara J Bond

Oregon State University;Dept. of Forest Science;330 Richardson Hall Corvallis OR 97331 USA

(541) 737-6110 (541) 908-2515 barbara.bond@oregonstate.edu Claire Phillips

USA

Claire.Phillips@oregonstate.edu claire.phillips@teraglobalchange.org Claire Phillips

USA

Claire.Phillips@oregonstate.edu claire.phillips@teraglobalchange.org Fox Sparky Peterson 404-580-5016 fox@tinybike.net Barbara J Bond

Oregon State University;Dept. of Forest Science;330 Richardson Hall Corvallis OR 97331 USA

(541) 737-6110 (541) 908-2515 barbara.bond@oregonstate.edu Principal Investigator Fox Sparky Peterson 404-580-5016 fox@tinybike.net Abstractor Barbara J Bond

Oregon State University;Dept. of Forest Science;330 Richardson Hall Corvallis OR 97331 USA

(541) 737-6110 (541) 908-2515 barbara.bond@oregonstate.edu Creator Claire Phillips

USA

Claire.Phillips@oregonstate.edu claire.phillips@teraglobalchange.org Creator 2013-08-16 Comparisons of respiration rate and environmental variables for mat and non-mat soil were conducted between July 2008 to Nov 2009 in a 0.1ha plot adjacent lower Lookout Creek, approximately 700m downstream from Lookout Camp (44 deg 13”25’N, 122 deg 15”30’W, 484m above sea level). The predominant overstory species are Psuedotsuga menziesii, Tsuga heterophylla, and Thuja Plicata. Associated ectomycorrhizal communities were measured over the 1.5 year period and data collection for the study is complete. Soil respiration was measured using LiCOR instrumentation, and analyses were performed computationally by correlating soil respiration with known environmental metrics (moisture, temperature, etc.) measured in other studies (TW006, MV001, etc.). populations carbon cycling soil fungi microbes LTER controlled vocabulary populations LTER core research areas Related PublicationsPhillips, Claire L. 2009. Distinguishing biological and physical controls on soil respiration. Corvallis, OR: Oregon State University. 137 p. Ph.D. dissertation.Phillips, C. L., Kluber, L. A., Martin, J. P., Caldwell, B. A., and Bond, B. J. 2012. Contributions of ectomycorrhizal fungal mats to forest soil respiration, Biogeosciences, 9, 2099-2110, doi:10.5194/bg-9-2099-2012. Data Use Agreement: The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. This Data Set is released under the Creative Commons license CC BY "Attribution" (see: https://creativecommons.org/licenses/by/4.0/). Creative Commons license CC BY - Attribution is a license that allows others to distribute, remix, tweak, and build upon your work (even commercially), as long as you are credited for the original creation. This license accommodates maximum dissemination and use of licensed materials. It is considered professional conduct and an ethical obligation to acknowledge the work of other scientists. The Data User is asked to provide attribution of the original work if this data package is shared in whole or by individual parts or used in the derivation of other products. A recommended citation is provided for each Data Set in the Andrews LTER data catalog (see: http://andlter.forestry.oregonstate.edu/data/catalog/datacatalog.aspx). A generic citation is also provided for this Data Set on the website https://portal.edirepository.org in the summary metadata page. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed and all data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. General acknowledgement: Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. http://andlter.forestry.oregonstate.edu/data/abstract.aspx?dbcode=SP033 A 0.1ha plot at HJ Andrews, along lower Lookout Creek, approximately 700m downstream from Lookout Camp, 44 deg 13”25’N, 122 deg 15”30’W, 484m above sea level -122.25833000 -122.25833000 44.22361000 44.22361000 2008-07-01 2009-11-30 All Organisms All Organisms Highest common category (ca. kingdom) Fungi The purpose of this study was to determine if there was an the incremental increase in soil respiration associated with the formation, function, and persistence of rhizomorphic ectomycorrhizal mats, and to determine which environmental factors (temperature, moisture, etc.) had direct influences on soil respiration. Knowing the patterns and drivers of soil respiration from heterotrophs is key to understanding forest function and the connectivity between soil and vegetative organisms.

An update history is logged and maintained with each new version of every dataset.

notPlanned Study code and preliminary metadata established Version1 2011-01-18 Uploaded all 4 entities into SQL. Version2 2013-08-06 Information Manager

Andrews Forest LTER Program US Forest Service Pacific Northwest Research Station 3200 SW Jefferson Way Corvallis OR 97331

hjaweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Claire Phillips

USA

Claire.Phillips@oregonstate.edu claire.phillips@teraglobalchange.org Andrews Forest LTER Site

Forest Ecosystems and Society Department in Forestry Oregon State University 201K Richardson Hall Corvallis OR 97331-5752

(541) 737-8480 lterweb@fsl.orst.edu http://andrewsforest.oregonstate.edu/ Field Methods - SP033: Soil respiration and temp Entity 1 (soil respiration and temperature): Twelve locations for respiration measurements were choosen randomly from approximately 20 suitable locations identified in an initial survey of mat coverage. Rhizomorphic mats were the most abundant type of EcM mat identified in the initial survey (hydrophobic mats were uncommon) and only rhizomorphic mats were selected for permanent measurements. Soil temperature was measured concurrently with respiration, using a Licor 6400 portable photosynthesis instrument with a LI-6400-19 soil chamber and a 10cm soil thermocouple probe. Respiration measurements were made with soil collars, which were inserted into the soil 1-2cm and left in place throughout the study. A few measurement locations were moved to accommodate recession or colonization of mats over time, and are noted in the data. Field Methods - SP033: Gravimetric soil moisture Entity 2 & 3 (gravimetric soil moisture): Soil moisture was measured gravimetrically in the Oi+Oe horizons, and at 5 and 15cm below the mineral soil surface, from cores taken at 6 small coring fields established across the site. Bulk density measured once at the beginning of the study was used to convert gravimetric measurements to volumetric water content at 5 and 15cm depth. A lookup table indicates the nearest respiration plots to each coring field. Field Methods - SP033: Soil cores Entity 4 (soil core data): At the outset of the study in July 2007, 10 Piloderma mat and 5 non mat soils (not paired) were choosed for one time respiration measurements (THESE ARE DIFFERENT LOCATIONS FROM LONGTERM RESPIRATION MEASUREMENTS). Soil cores were sampled destructively to measure root biomass, %C and %N, soil pH, moisture, and litter depth. Respiration rates were also measured prior to coring. Soils cores 8cm in diameter were removed in 4 depth increments: the entire O-horizon, 0-10cm, 10-20cm, and 20-35cm below the mineral soil surface. Fine root (less than 2mm diameter) and total root biomass were determined by wet sieving soils through 1mm sieves, and picking roots by hand. Soil pH was measured in wet soil slurries with distilled water. We measured total soil C and N by drying 1g of organic soil and 5g of mineral soil at 65°C for 48 hours, grinding soils to fine powder on a roller mill, and analyzing 3-10mg subsamples on a Costech ECS-4010 elemental combustion analyzer (Costech Analytical, Valencia, CA, USA) against an atropine standard. Laboratory Methods - SP033 Entity 5: We assessed the presence of Piloderma by comparing T-RFLP patterns from soil extracts to those of known Piloderma isolates. Detailed methods of extraction and analysis are described by Blanchard (2008, M.S. Thesis, Oregon State University). Soil samples were kept cold on ice in the field and frozen at -80ºC upon returning to the lab. Three grams of frozen soil were ground with mortar and pestle to homogenize, and 0.25g of ground soil was used to extract DNA with the MOBio PowerSoilTM DNA isolation kit (MoBio Laboratories, Carlsbad, CA). We amplified fungal DNA using ITS1-F and ITS4 rDNA primers, with the forward primers fluorescently labeled with 5’-6-FAM (6-carboxyfluorescein). PCR products were cleaned to remove excess primer and nucleotides, and then digested with Hinf1 restriction endonucleases. The restriction products were submitted to OSU Center for Genome Research and Biocomputing for analysis. Size and relative abundance of the T-RFLP fragments were quantified using Genotyper® version 3.7 software (Applied Biosystems, Inc., Foster City, CA). Site was a 0.1ha area along lower Lookout Creek, approximately 700m downstream from Lookout Camp. Area was also used in the microbial observatory study (MO site 17). The forest stand was ~450 years old, dominated by Douglas-fir (Psuedotsuga menziesii) and western hemlock (Tsuga heterophylla), both hosts for mat-forming EcM species, and western redcedar (Thuja plicata), a host for arbuscular mycorrhizal fungi, which do not form mats. The understory plants included Acer circinatum, Gaultheria shallon, Vaccinium parvifolium, Polystichum munitum, Oxalis oregano, and the forest floor was covered with a layer of bryophytes. Fallen logs in advance stages of decay were common. The soil has strong andic properties and is classified as coarse loamy mixed mesic Typic Hapludands (Dixon, 2003), with an O-horizon depth of 4-9cm. Sampling frequency: Every 2-4 weeks spring-fall Repeated measurements of respiration rate, soil moisture, and temperature were taken every 2-4 weeks at 12 pairs of mat and neighboring non-mat soils from July 2008 to December 2009 while the ground was free of snow. Sherri L. Johnson

US Forest Service ;Pacific NW Research Station ;3200 SW Jefferson Way Corvallis OR 97331 USA

541-758-7771 sherri.johnson2@usda.gov sherri.johnson@oregonstate.edu https://www.fs.fed.us/research/people/profile.php?alias=sherrijohnson Principal Investigator Julia A. Jones

Oregon State University;Department of Geosciences; Wilkinson Hall 104 Corvallis OR 97331-5506 USA

(541) 737-1224 Julia.Jones@oregonstate.edu geojulia@comcast.net http://ceoas.oregonstate.edu/profile/jones/ http://orcid.org/0000-0001-9429-8925 Principal Investigator Matthew G Betts

Department of Forest Ecosystems and Society; 201E Richardson Hall; College of Forestry; Oregon State University Corvallis OR 97331

(541) 737-3841 matt.betts@oregonstate.edu http://www.fsl.orst.edu/flel/index.htm Principal Investigator Michael P. Nelson

Department of Forest Ecosystems and Society; 201K Richarson Hall; College of Forestry; Oregon State University Corvallis OR 97331

541-737-9221 mpnelson@oregonstate.edu http://www.michaelpnelson.com http://orcid.org/0000-0001-6917-4752 Principal Investigator David Bell david.bell@usda.gov david.bell@oregonstate.edu https://lemma.forestry.oregonstate.edu/about/david-bell Principal Investigator The H.J. Andrews Experimental Forest is a living laboratory that provides unparalleled opportunities for the study of forest and stream ecosystems in the central Cascade Range of Oregon. Since 1980, as a part of the National Science Foundation Long Term Ecological Research (NSF-LTER) program, the Andrews Experimental Forest has become a leader in the analysis of forest and stream ecosystem dynamics. Long-term field experiments and measurement programs have focused on climate dynamics, streamflow, water quality, and vegetation succession. Currently researchers are working to develop concepts and tools needed to predict effects of natural disturbance, land use, and climate change on ecosystem structure, function, and species composition. The Andrews Experimental Forest is administered cooperatively by the USDA Forest Service Pacific Northwest Research Station, Oregon State University and the Willamette National Forest. Funding for the research program comes from the National Science Foundation (NSF), US Forest Service Pacific Northwest Research Station, Oregon State University, and other sources. Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. National Science Foundation: DEB1440409 The Andrews Forest is situated in the western Cascade Range of Oregon, and covers the entire 15,800-acre (6400-ha) drainage basin of Lookout Creek. Elevation ranges from 1350 to 5340 feet (410 to 1630 m). Broadly representative of the rugged mountainous landscape of the Pacific Northwest, the Andrews Forest contains excellent examples of the region's conifer forests and associated wildlife and stream ecosystems. These forests are among the tallest and most productive in the world, with tree heights of often greater than 250 ft (75 m). Streams are steep, cold and clean, providing habitat for numerous aquatic organisms. 1 SP03301 Soil respiration rates and temperature: SP03301.csv 12545 bfdd8f6bb753b1d1e1bac194d81e1573 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP03301_v1.csv 2008-07-01 2009-11-01 DBCODE Database Code char(5) SP033 FSDB Database code SP033 ENTITY Entity number numeric(2,0) number 1 natural 1.0000 1.0000 DATE Date of measurement datetime YYYY-MM-DD 7/7/2007 12:00:00 AM 10/30/2008 12:00:00 AM RESP_PLOT ID for mat and non-mat pair (longterm measurement plot) char(8) PAIR_01 Mat/non-mat pair 1 PAIR_02 Mat/non-mat pair 2 PAIR_03 Mat/non-mat pair 3 PAIR_04 Mat/non-mat pair 4 PAIR_05 Mat/non-mat pair 5 PAIR_06 Mat/non-mat pair 6 PAIR_07 Mat/non-mat pair 7 PAIR_08 Mat/non-mat pair 8 PAIR_09 Mat/non-mat pair 9 PAIR_10 Mat/non-mat pair 10 PAIR_11 Mat/non-mat pair 11 PAIR_12 Mat/non-mat pair 12 PAIR_07b Mat/non-mat pair 7 (new) PAIR_07a Mat/non-mat pair 7 (old) MAT_NON Mat or non-mat soil type char(1) M Mat soil type N Non-mat soil type EFFLUX_AVG Average efflux from 2 or 3 measurements in immediate succession numeric(7,3) micromoles per square meter per second 0.001000 real 0.0000 15.0000 EFFLUX_SE Standard error of mean numeric(6,3) micromoles per square meter per second 0.001000 real 0.0000 2.0000 CO2_AVG Initial CO2 concentration in soil chamber numeric(6,1) parts per million 0.100000 real 370.0000 512.0000 TEMP_10CM Soil temperature at 10cm depth numeric(5,2) degrees Celsius 0.100000 real -10.0000 40.0000 LOCATION_NOTES Notes regarding relocation of measurement varchar(75) Notes regarding relocation of measurement PRIMARY SP03301.DATE SP03301.MAT_NON SP03301.RESP_PLOT NOTNULL SP03301.CO2_AVG SP03301.DATE SP03301.DBCODE SP03301.EFFLUX_AVG SP03301.ENTITY SP03301.MAT_NON SP03301.RESP_PLOT SP03301.TEMP_10CM 211 2 SP03302 Gravimetric soil moisture corresponding with respiration: SP03302.csv 4739 1c78e7361156673d981a8a49b63d3d4f 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP03302_v1.csv 2008-07-01 2009-11-01 DBCODE Database Code char(5) SP033 FSDB Database code SP033 ENTITY Entity number numeric(2,0) number 1 natural 2.0000 2.0000 GWC_AREA Coring field area char(6) AREA_1 Core field area 1 AREA_2 Core field area 2 AREA_3 Core field area 3 AREA_4 Core field area 4 AREA_5 Core field area 5 DATE Date of measurement datetime YYYY-MM-DD 7/7/2007 12:00:00 AM 10/30/2008 12:00:00 AM GWC_LITTER Gravimetric water content of Oi and Oe horizons (g water per g dry soil) numeric(6,2) grams per gram 1 real 19.4100 340.8400 GWC_5CM Gravimetric water content 5cm below mineral soil surface (g water per g dry soil) numeric(5,2) grams per gram 1 real 23.7600 98.6500 GWC_15CM Gravimetric water content 15cm below mineral soil surface (g water per g dry soil) numeric(5,2) grams per gram 1 real 23.4200 52.0800 PERC_WC_LITTER Litter water content expressed as a percent of total weight numeric(3,0) percent 1 whole 16.0000 77.0000 EST_VWC_5CM Estimated volumetric water content at 5cm using a bulk density of 0.75 numeric(5,2) cubic meters per cubic meter 1 real 17.8200 73.9900 EST_VWC_15CM Estimated volumetric water content at 15cm using a bulk density of 0.78 numeric(6,2) cubic meters per cubic meter 1 real 18.2700 143.7700 PRIMARY SP03302.DATE SP03302.GWC_AREA NOTNULL SP03302.DATE SP03302.DBCODE SP03302.ENTITY SP03302.EST_VWC_15CM SP03302.EST_VWC_5CM SP03302.GWC_15CM SP03302.GWC_5CM SP03302.GWC_AREA SP03302.GWC_LITTER SP03302.PERC_WC_LITTER 74 3 SP03303 Gravimetric soil moisture lookup table: Lookup table associates soil moisture coring fields with closest respiration plots SP03303.csv 370 7926ac276acd9ec7827a2263af6048db 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP03303_v1.csv 2008-07-01 2009-11-01 DBCODE Database Code char(5) SP033 FSDB Database code SP033 ENTITY Entity number numeric(2,0) number 1 natural 3.0000 3.0000 RESP_PLOT ID for mat and non-mat pair (longterm measurement plot) char(8) PAIR_01 Mat/non-mat pair 1 PAIR_02 Mat/non-mat pair 2 PAIR_03 Mat/non-mat pair 3 PAIR_04 Mat/non-mat pair 4 PAIR_05 Mat/non-mat pair 5 PAIR_06 Mat/non-mat pair 6 PAIR_07 Mat/non-mat pair 7 PAIR_08 Mat/non-mat pair 8 PAIR_09 Mat/non-mat pair 9 PAIR_10 Mat/non-mat pair 10 PAIR_11 Mat/non-mat pair 11 PAIR_12 Mat/non-mat pair 12 PAIR_07b Mat/non-mat pair 7 (new) PAIR_07a Mat/non-mat pair 7 (old) GWC_AREA Coring field area char(6) AREA_1 Core field area 1 AREA_2 Core field area 2 AREA_3 Core field area 3 AREA_4 Core field area 4 AREA_5 Core field area 5 PRIMARY SP03303.GWC_AREA SP03303.RESP_PLOT NOTNULL SP03303.DBCODE SP03303.ENTITY SP03303.GWC_AREA SP03303.RESP_PLOT 12 4 SP03304 Soil evironmental and physical characteristics: SP03304.csv 7691 bedccb276c2be66cf6fb853be31e93f0 1 \r\n column , " http://andlter.forestry.oregonstate.edu/ltermeta/ltersearch/dataaccess.aspx?docid=SP03304_v1.csv 2008-07-30 2008-07-30 DBCODE Database Code char(5) SP033 FSDB Database code SP033 ENTITY Entity number numeric(2,0) number 1 natural 4.0000 4.0000 DATE Date of measurement datetime YYYY-MM-DD 7/30/2008 12:00:00 AM 7/30/2008 12:00:00 AM MAT_NON Mat or non-mat soil type char(1) M Mat soil type N Non-mat soil type CORE_ID ID for soil core (different from respiration plot) char(2) CORE_01 Soil core 1 CORE_02 Soil core 2 CORE_03 Soil core 3 CORE_04 Soil core 4 CORE_05 Soil core 5 CORE_06 Soil core 6 CORE_07 Soil core 7 CORE_08 Soil core 8 CORE_09 Soil core 9 CORE_10 Soil core 10 DEPTH Depth of core char(6) litter Oe,Oi, and Oa layers m00 mineral soil surface to 10cm below m10 10-20cm below the mineral soil surface m20 20-35cm below the mineral soil surface LITTER_DEPTH Total depth (cm) of Oi, Oe, and Oa layers. numeric(4,1) centimeters 0.100000 real 2.4000 17.5000 CORE_AREA Cross-sectional area of core (cm2) numeric(4,1) square centimeters 0.010000 real 50.2000 78.5000 CORE_VOL Total volume of core (cm3) numeric(7,2) cubic centimeters 0.010000 real 188.4000 1373.7500 SOIL_WET_MASS Total mass of field-moist core (g) numeric(6,1) grams 0.100000 real 20.0000 1170.0000 PH pH numeric(4,2) pH units 0.010000 real 3.8100 6.2600 GWC Gravimetric water content (g water/g dry soil) numeric(6,4) grams per gram 0.000100 real 0.3094 1.5659 PERCENTN Total nitrogen content, by mass numeric(4,2) percent 0.010000 real 0.1000 1.7300 PERCENTC Total carbon content, by mass numeric(5,2) percent 0.010000 real 2.1500 48.4100 CTON_RATIO Carbon to nitrogen ratio numeric(5,2) number 0.010000 real 19.5400 67.5900 FINE_ROOTS Mass of roots less than 2mm diameter (g water/g dry soil) numeric(6,4) grams per gram 0.000100 real 0.1407 3.2688 THIN1_ROOTS Mass of roots 2-4 mm diameter (g water/g dry soil) numeric(6,4) grams per gram 0.000100 real 0.0000 1.9816 THIN2_ROOTS Mass of roots 4-6mm diameter (g water/g dry soil) numeric(6,4) grams per gram 0.000100 real 0.0000 1.9834 THIN3_ROOTS Mass of roots 6-8mm diameter (g water/g dry soil) numeric(6,4) grams per gram 0.000100 real 0.0000 4.1700 COARSE_ROOTS Mass of roots >8mm diameter (g water/g dry soil) numeric(7,4) grams per gram 0.000100 real 0.0000 21.9900 TOTAL_ROOTS Total root mass (g water/g dry soil) numeric(7,4) grams per gram 0.000100 real 0.3266 25.2000 EFFLUX_AVG Average efflux from 2 or 3 measurements in immediate succession numeric(7,3) micromoles per square meter per second 0.001000 real 2.1340 12.0300 EFFLUX_SE Standard error of mean numeric(6,3) micromoles per square meter per second 0.001000 real 0.0780 1.1000 CO2_AVG Initial CO2 concentration in soil chamber numeric(6,1) parts per million 0.100000 real 425.0000 546.5000 TEMP_10CM Soil temperature at 10cm depth numeric(5,2) degrees Celsius 0.100000 real 12.9000 15.9000 PRIMARY SP03304.CORE_ID SP03304.DATE SP03304.DEPTH SP03304.MAT_NON NOTNULL SP03304.CORE_AREA SP03304.CORE_ID SP03304.CORE_VOL SP03304.DATE SP03304.DBCODE SP03304.DEPTH SP03304.ENTITY SP03304.MAT_NON SP03304.SOIL_WET_MASS 60 cubic meters per cubic meter parts per million square centimeters cubic centimeters micromoles per square meter per second dimensionless number, i.e., ratio, count centimeters; .01 meters percent; a number Scale used for pH measurements grams; 0.001 kilogram Degrees Celsius; a common unit of temperature; constantToSI=273.18 grams per gram (often in terms of dry weight)