• Sherri L. Johnson - Principal Investigator
  US Forest Service ;Pacific NW Research Station ;3200 SW Jefferson Way, Corvallis, OR, 97331, USA
  Phone: 541-758-7771
  Email: sherri.johnson2@usda.gov, sherri.johnson@oregonstate.edu
  URL: https://www.fs.fed.us/research/people/profile.php?alias=sherrijohnson
• Julia A. Jones - Principal Investigator
  Oregon State University;Department of Geosciences; Wilkinson Hall 104, Corvallis, OR, 97331-5506, USA
  Phone: (541) 737-1224
  Email: Julia.Jones@oregonstate.edu, geojulia@comcast.net
  URL: http://ceoas.oregonstate.edu/profile/jones/
  ORCID: http://orcid.org/0000-0001-9429-8925
• Matthew G Betts - Principal Investigator
  Department of Forest Ecosystems and Society; 201E Richardson Hall; College of Forestry; Oregon State University, Corvallis, OR, 97331
  Phone: (541) 737-3841
  Email: matt.betts@oregonstate.edu
  URL: http://www.fsl.orst.edu/flel/index.htm
• Michael P. Nelson - Principal Investigator
  Department of Forest Ecosystems and Society; 201K Richarson Hall; College of Forestry; Oregon State University, Corvallis, OR, 97331
  Phone: 541-737-9221
  Email: mpnelson@oregonstate.edu
  URL: http://www.michaelpnelson.com
  ORCID: http://orcid.org/0000-0001-6917-4752
• David Bell - Principal Investigator
  Email: david.bell@usda.gov, david.bell@oregonstate.edu
  URL: https://lemma.forestry.oregonstate.edu/about/david-bell

• The H.J. Andrews Experimental Forest is a living laboratory that provides unparalleled opportunities for the study of forest and stream ecosystems in the central Cascade Range of Oregon. Since 1980, as a part of the National Science Foundation Long Term Ecological Research (NSF-LTER) program, the Andrews Experimental Forest has become a leader in the analysis of forest and stream ecosystem dynamics.
• Long-term field experiments and measurement programs have focused on climate dynamics, streamflow, water quality, and vegetation succession. Currently researchers are working to develop concepts and tools needed to predict effects of natural disturbance, land use, and climate change on ecosystem structure, function, and species composition.
• The Andrews Experimental Forest is administered cooperatively by the USDA Forest Service Pacific Northwest Research Station, Oregon State University and the Willamette National Forest. Funding for the research program comes from the National Science Foundation (NSF), US Forest Service Pacific Northwest Research Station, Oregon State University, and other sources.

Data were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 2025755), US Forest Service Pacific Northwest Research Station, and Oregon State University. National Science Foundation: DEB1440409

• Long-Term Ecological Research
  The Andrews Forest is situated in the western Cascade Range of Oregon, and covers the entire 15,800-acre (6400-ha) drainage basin of Lookout Creek. Elevation ranges from 1350 to 5340 feet (410 to 1630 m). Broadly representative of the rugged mountainous landscape of the Pacific Northwest, the Andrews Forest contains excellent examples of the region's conifer forests and associated wildlife and stream ecosystems. These forests are among the tallest and most productive in the world, with tree heights of often greater than 250 ft (75 m). Streams are steep, cold and clean, providing habitat for numerous aquatic organisms.

• Sediments were sampled on 4 occasions: 6/26, 7/1, 7/30, 9/6 1996. FBOM was collected from stream beds with a hand vacuum pump into a 2 L collecting jar. The intake line was fitted with a 1 mm stainless steel screen, allowing benthic material to be wet-sieved during sampling. Samples were transferred to polystyrene jars (500 mL) and stored in an insulated chest with stream water and ice. In the laboratory, a slurry was prepared by decanting excess stream water from the jars and mixing, keeping FBOM suspended while subsampling. Subsamples were dispensed using 1, 3, or 5 mL plastic syringes with enlarged openings. All laboratory analyses of slurries began immediately upon return from FBOM collection (Bonin et al., 2000) conform with known time constraints on sampling and sample processing (Bonin et al., 1999).

• Denitrification potential was measured as NO production in FBOM slurries incubated in an AR atmosphere and amended with glucose and NaNO (Martin et al. 1988). Duplicate 5 mL FBOM slurry samples in 25 mL Erlenmeyer flasks were capped with rubber stoppers and purged for 3 minutes with argon at 120 cc/min. The flasks were gently shaken to remove air bubble and incubated at 24°C for 1h. After this initial incubation, 2 mL of a sterile 1 mM glucose and 1 mM NaNO solution was injected through the stopper and 2 mL of headspace removed. Incubation was continued at 24°C for an additional 3 h. After 1 and 3 hours, a gas chromatograph (GC) equipped with an electron capture detector was used to measure NO concentrations.
• Putative nitrogen fixation rates were measured by acetylene reduction (Weaver and Danso 1994). Samples were prepared as for denitrification except the headspace was replaced with 1.5% O, 12.5% acetylene and 86% argon. After the samples had incubated for 24 h, ethylene concentrations were measured on a GC equipped with a flame ionization detector. A control without acetylene was analyzed for endogenous ethylene production.
• Respiration was measured on duplicate 5 mL FBOM slurry samples in 25 mL Erlenmeyer flasks capped with rubber stoppers. Slurries were incubated at 24°C for 3 h. At 1 and 3 h., the headspace was analyzed for CO on a GC fitted with a thermal conductivity conductor.
• s-glucosidase activities were measured using the spectrophotometric assay of Tabatabai and Bremner (1969), as modified by Zou et al. (1992). One mL of 10 mM p-nitrophenyl s-D glucopyranoside substrate was added to duplicate 1 mL subsamples containing suspended FBOM. The tubes were shaken and then placed in a 30°C water bath for 2 hours, along with duplicate controls with no s-glucosidase substrate addition. After incubating, 1 mL of 10 mM p-nitrophenyl s-D glucopyranoside was added to the controls and all reactions were immediately stopped with the addition of 0.5 mL of 0.5 M CaCl and 2 mL of 0.1 M tris[hydroxymethyl]aminomethane at pH 12.0. The mixtures were centrifuged for 5 minutes at 500 x g. From the supernatant, 0.2 mL of solution was diluted with 2.0 mL deionized water and the optical density measured at 410 nm. A standard curve was prepared from 0.02-1.0 Fmol mL p-nitrophenol. Phosphatase followed the same general procedure as for s-glucosidase, except the substrate used was 1 mL of 50 mM p-nitrophenyl phosphate, incubation period was 1 hour, and 2 mL of 0.5 M NaOH instead of 0.1 M tris[hydroxymethyl]aminomethane, were added to terminate the reaction.
• Mineralizable nitrogen measurements were determined using the 7 d anaerobic incubation method (Keeney 1982). Duplicate 10 mL FBOM samples in 50 mL screw-topped test tubes were filled to the top edge with deionized water, capped and incubated at 40°C for 7 d. After incubation an equal amount of 4 M KCl was added, shaken for 1 hour in the presence of 0.4 mL 10M NaOH and analyzed for NH-N using a selective ion electrode (Corning ammonium electrode, Medford, MA). The value for extractable ammonium was subtracted from the total to account for background levels of ammonium prior to incubation.
• Extractable ammonium was extracted by adding 50 mL of 2 M KCl to duplicate 10 mL samples in 250 mL Erlenmeyer flasks. Flasks were capped, shaken while incubating for 1 hour in the presence of 0.4 mL 10 M NaOH and analyzed using a selective ion electrode to determine KCl-extractable ammonium concentration.

• Sampling frequency: Four monthly samples at each site.

• We wanted to determine the effects of recent debris flows on the biochemical characteristics of small stream fine benthic organic matter (FBOM). Seven pairs of streams were used; half had experienced debris flows during the late winter storms and half did not.

• Andrews Experimental Forest (HJA)
  W -122.26172200, E -122.10084700, N 44.28196400, S 44.19770400
  Altitude: 1631 to 1631 meter

Object name: HS00601.csv

Records: N/A

Attributes: 22

Temporal coverage: 1996-06-26 to 1996-09-06

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